Microscope Incubators
IVF equipment
Gas Mixers
The Water Jacket Incubator provides superior incubation stability above and below room temperature. Water is pumped from a water bath into the base plate and into the lid, which are both Water Jackets.Temperature is measured inside a reference Petri-dish and is controlled by circulating water from a water bath into the Water Jacket Chamber. The Temperature Controller reads the temperature in the reference well and updates the set point temperature of the water bath, ensuring a specimen temperature stability of ± 0.1°C.
SELECT THE CLIMATE CHAMBER THAT FITS YOUR XY STAGE / Z PIEZO
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
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| Applied Precision | 51-603288-000 Deltavision Stage | H101-K-FRAME | DV-PA | ||||||||||||||||||||
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| Mad City Labs | Z100/Z500 Piezo stages | H101-MCL-Z100/500 | - | ||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H101-K-FRAME | - | ||||||||||||||||||||
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H101-K-FRAME | THORPA | ||||||||||||||||||||
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| Upright Microscopes | any stage for uprightmicroscopes | H101-UP | - | ||||||||||||||||||||
The Water Jacket Incubator provides superior incubation stability above and below room temperature by circulating water inside the base and lid of the water jacket chamber. Feedback is provided by sample temperature or by chamber temperature. Selection is done via OKO-TOUCH.
Okolab offers two Temperature controllers, according to the temperature range of interest.
From 3 above ambient to 60°C
From -5°C to 60°C
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
A humidity module based on a bubbling column is typically used with a Heating and Cooling System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Oxygen control is our pride.
Check out the available solutions in the Controller List
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 0-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
These gas mixers employ accurate floating ball flow meters to mix gas AIR and CO2 streams. The desired gas concentration is achieved by measuring the individual flow rated prior to mixing.
For Hypoxia or Hyperoxia applications, a third flow meter if added to measure N2 or O2 flow rate.
No feedback is present on gas concentration.
All you need is a single flow meter to regulate the flux
3GF-MIXER is a simple and cost effective solution also for these application.
Use AIR-PUMP to feed air into the Manual Gas Mixers
Available Manual Gas Mixers
| 2GF MIXER | 0-20% | - | 0 |
| 3GF MIXER-HYPOXIA | 0-15% | 2-20% | 0 |
| 3GF MIXER-HYPEROXIA | 0-15% | 40-85% | 0 |
The Heating and Cooling Stage Top Incubator employes a bubbling column to humidify the gas prior to the inlet inlet in the stage top chamber.
The bubbling column is immersed in the water bath and a pre-heating coil ensures thermal equilibrium between the flowing gas and the water bath.
The tube connecting the bubbling column to the stage top chamber is isolated to prevent water condensation.
The system comprises:
Active Humidity Controller controls the Relative Humidity inside the chamber in the range 50-95% with a resolution of 1%.
The dry gas is humidified by flowing over warm water. The Humidity sensor regulates water temperature in order to achieve the desired relative humidity in the chamber.
To prevent water condensation, the tube connecting the humidity controller to the chamber is heated.
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX (that also allows remote control), via DATA-LOG Okolab software (that also allows to set enhanced thermal/gas cycles), and via following third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN Black software is available upon request.
Smart Box connects to the Bold Line Controller and provides the following features:
The microscope enclosure creates a large volume around the microscope in which temperature is controlled by recirculating warm air. It is made of polycarbonate panels and encloses microscope stage, nosepiece and objectives. It doesn't affect focus stability. All electronics including EMCCDs, spinning disk confocal etc. are left outside of the enclosure avoiding exposure to 37°C.
The enclosure closely follows the microscope profile and is supported by posts resting on the vibration isolation table.
Take the front panel off !
Make the enclosure dark !
- Enclosure
- Temperature Controller
- Gas Chamber
- CO2 Digital Controller
- Vibration Free Humidity Module
Okolab Cage Incubator employs a Temperature Unit that generates warm air to raise the temperature inside the enclosure.
Superior accuracy is guaranteed by the interplay of four sensors: three for temperature and one for flow rate. The temperature sensors monitor temperature inside the enclosure, inside the Gas Micro-Environmental Chamber and inside the Air Heater. The flow rate sensor controls the rate at which warm air enters the enclosure and ensures smooth operation and fast heating. A proprietary smart algorithm determines the minimum flow rate necessary to maintain the controlled temperature at the set point value.
Okolab Cage Incubation System has both Enclosure (Chamber) Feedback mode as well as Sample Feedback mode as standard features. The end user can select the temperature sensor driving feedback to the controller.
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
It measures air temperature inside the enclosure.
It measures the temperature in a reference well near the sample.
It ensures Temperature stability even if the doors of the enclosure are temporarely opened.
The micro-environmental Gas Chamber fits on the microscope stage and hosts the sample. The Gas Chamber is located inside the enclosure and at the set point temperature - typically 37°C. The purpose for the Gas Chamber is to create a small volume surrounding the sample and containing humidified gas of the desired concentration.
When operating the Temperature Unit in Sample Feedback Mode, introduce the provided temperature sensor into the Gas Chamber through the temperature sensor port or through one of the built-in perfusion holes and position it in a reference well.
Remove the chamber riser and use the Koehler Lid.
Each chamber features up to 12 holes for inlet and outler of perfusion tubings
Change the chamber insert. It is a 5 seconds task with the new magnetic sample holders
Keep the sample in the correct position by using the Magnetic Locks
Chambers available for any XY stage / piezo insert
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Applied Precision | 51-603288-000 Delta vision Stage | H201-K-FRAME | DV-PA | ||||||||||||||||||||||||
| ASI | 5MS-2000, MS-2000 FT, PZ-2000, PZ-2000FT | H201-K-FRAME | - | ||||||||||||||||||||||||
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| Mad City Labs | Z100/Z500 Piezo stages | H201-MCL-Z100/500 | - | ||||||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H201-K-FRAME | - | ||||||||||||||||||||||||
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H201-K-FRAME | THORPA | ||||||||||||||||||||||||
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| Upright Microscopes | any stage for uprightmicroscopes | H201-UP | - | ||||||||||||||||||||||||
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
A vibration free humidity module is typically used with a Cage Incubation System. When Humidity control with feedback is required, the Active Humidity Controller can be connected to the Bold Line Gas Controller.
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Oxygen control is our pride.
Check out the available solutions in the Controller List
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 0-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
These gas mixers employ accurate floating ball flow meters to mix gas AIR and CO2 streams. The desired gas concentration is achieved by measuring the individual flow rated prior to mixing.
For Hypoxia or Hyperoxia applications, a third flow meter if added to measure N2 or O2 flow rate.
No feedback is present on gas concentration.
All you need is a single flow meter to regulate the flux
3GF-MIXER is a simple and cost effective solution also for these application.
Use AIR-PUMP to feed air into the Manual Gas Mixers
Available Manual Gas Mixers
| 2GF MIXER | 0-20% | - | 0 |
| 3GF MIXER-HYPOXIA | 0-15% | 2-20% | 0 |
| 3GF MIXER-HYPEROXIA | 0-15% | 40-85% | 0 |
The Cage Incubator can be equipped with a vibration-free gas humidifier which employes a semi-permeable membrane. Humidification occurs without bubbling to ensure vibration-free operation.
A water trap is located between the humidifier and the gas chamber preventing any water droplet from accidentally reaching the sample.
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX (that also allows remote control), via DATA-LOG Okolab software (that also allows to set enhanced thermal/gas cycles), and via following third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN Black software is available upon request.
Smart Box connects to the Bold Line Controller and provides the following features:
The Stage Top Chambers fits in the XY stage or Piezo insert of the microscope, connects to Okolab Temperature, Gas and Humidity Controllers and creates the proper environment for live cell imaging right on the microscope stage.
Remove the chamber riser and use the Koehler Lid.
Each chamber features up to 12 holes for inlet and outler of perfusion tubings
Change the chamber insert. It is a 5 seconds task with the new magnetic sample holders
Keep the sample in the correct position by using the Magnetic Locks
Chambers available for any XY stage / piezo insert
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Applied Precision | 51-603288-000 Deltavision Stage | H301-K-FRAME | DV-PA | ||||||||||||||||||||
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| Mad City Labs | Z100/Z500 Piezo stages | H301-MCL-Z100/500 | - | ||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H301-K-FRAME | - | ||||||||||||||||||||
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H301-K-FRAME | THORPA | ||||||||||||||||||||
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| Upright Microscopes | any stage for uprightmicroscopes | H301-UP | - | ||||||||||||||||||||
H301-T-UNIT-BL-PLUS is the most advanced solution for Temperature Control. It is compatible with Digital Gas Controllers and with Active Humidity Controller to ensure best environmental control for live cell imaging.
H301-T-UNIT-BL-PLUS has five independent temperature channels: chamber base, chamber lid, objective heater, sample temperature sensor, room temperature sensor.
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
H301-T-UNIT-BL-PLUS is designed to perfectly fit with:
It measures the temperature in a reference well near the sample.
It ensures Temperature stability even if the doors of the enclosure are temporarely opened.
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
The Active Humidity Controller is typically used with a Stage Top Incubation System.
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Oxygen control is our pride.
Check out the available solutions in the Controller List
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 1-18% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
Active Humidity Controllers control the Relative Humidity inside the chamber in the range 50-95% with a resolution of 1%.
The dry gas is humidified by flowing over warm water. The Humidity sensor regulates water temperature in order to achieve the desired relative humidity in the chamber.
To prevent water condensation, the tube connecting the humidity controller to the chamber is heated.
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
Smart Box connects to the Bold Line Controller and provides the following features:
The Stage Top Chambers fits in the XY stage or PIezo insert of the microscope, connects to Okolab Temperature, Gas and Humidity Controllers and creates the proper environment for live cell imaging right on the microscope stage.
Remove the chamber riser and use the Koehler Lid.
Each chamber features up to 12 holes for inlet and outler of perfusion tubings
Change the chamber insert. It is a 5 seconds task with the new magnetic sample holders
Keep the sample in the correct position by using the Magnetic Locks
Chambers available for any XY stage / piezo insert
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Applied Precision | 51-603288-000 Deltavision Stage | H301-K-FRAME | DV-PA | ||||||||||||||||||||
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| Mad City Labs | Z100/Z500 Piezo stages | H301-MCL-Z100/500 | - | ||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H301-K-FRAME | - | ||||||||||||||||||||
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H301-K-FRAME | THORPA | ||||||||||||||||||||
|
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| Upright Microscopes | any stage for uprightmicroscopes | H301-UP | - | ||||||||||||||||||||
H301-T-UNIT-BL-PLUS is the most advanced solution for Temperature Control. It is compatible with Digital Gas Controllers and with Active Humidity Controller to ensure best environmental control for live cell imaging.
H301-T-UNIT-BL-PLUS has five independent temperature channels: chamber base, chamber lid, objective heater, sample temperature sensor, room temperature sensor.
Beautifully designed, simple to use and very compact.
OKO-TOUCH is all you need to operate the system.
H301-T-UNIT-BL-PLUS is designed to perfectly fit with:
It measures the temperature in a reference well near the sample.
It ensures Temperature stability even if the doors of the enclosure are temporarely opened.
The Bold Line Gas Controllers connect to the Temperature Controller via a cable-less connector and are operated through the same touch screen interface OKO-TOUCH.
CO2 and or O2 accuracy is ±0.1% with adjustable total output flow rate.
The Active Humidity Controller is typically used with a Stage Top Incubation System.
Use the best Humidifier on the market: ACTIVE HUMIDITY CONTROLLER
It connects to the Gas Controller and shows up in OKO-TOUCH
Oxygen control is our pride.
Check out the available solutions in the Controller List
One controller feeds up to 10 microscope incubators.
Microscopes can be in different rooms.
Available Bold Line Digital Controllers
| CO2 | O2 | Accuracy | Outlet P, barg (psi) | |
|---|---|---|---|---|
| CO2-Unit-BL | 0-18% | - | ±0.1% | 0 |
| CO2-Unit-BL-CP2 (multi-user centralized controller) | 0-10% | - | ±0.1% | 1.5 (22) |
| O2-Unit-BL [1-20] | - | 1-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10;1-18] | 0-10% | 0-20% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-20;1-95] | 0-20% | 1-95% | ±0.1% | 0 |
| CO2-O2 Unit-BL [0-10; 0-1] | 0-10% | 0-1% | ±0.1% | 0 |
Active Humidity Controllers control the Relative Humidity inside the chamber in the range 50-95% with a resolution of 1%.
The dry gas is humidified by flowing over warm water. The Humidity sensor regulates water temperature in order to achieve the desired relative humidity in the chamber.
To prevent water condensation, the tube connecting the humidity controller to the chamber is heated.
OKO-TOUCH is an intuitive and user-friendly touch screen interface. It connects to any one of the Bold Line Controllers, detects all equipment in the system and displays all functions supported by the equipment detected.
OKO-TOUCH features on-board memory for data logging and mini-USB port for data download.
In addition data logging is possible via SMART-BOX (that also allows remote control), via DATA-LOG Okolab software (that also allows to set enhanced thermal/gas cycles), and via following third-party Imaging software: LASX 2.0, NIS-Elements, SlideBook, MetaMorph and MicroManager. Macro for ZEN Black software is available upon request.
Smart Box connects to the Bold Line Controller and provides the following features:
UNO STAGE TOP INCUBATOR ensures the highest temperature accuracy on sample temperature by controlling simultaneously up to 6 independent channels with a modern multichannel controller.
The name "UNO" reflects the simplicity of the system, which allows temperature, humidity and CO2 conditioning in a very user friendly manner.
The controller is equipped with an on-board memory for data logging and a mini-USB port for data download.
Chambers available for any XY stage / piezo insert with Embedded temperature sensor in heated glass lid and in chamber body
UNO STAGE TOP INCUBATOR employs a powerful humidifier to ensure 90% ca. of relative humidity inside the stage top incubator
UNO-T-H-PREMIXED requires pre-mixed gas
UNO-T-H-CO2 provides gas mixer
Collars fit any objective on the market
Compatible with Inverted and Upright Microscopes
Chambers available for any XY stage / piezo insert
| Manufacturer | Model or stage opening | Climate Chamber | Fixing tool | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Applied Precision | 51-603288-000 Deltavision Stage | H301-K-FRAME | DV-PA | ||||||||||||||||||||
|
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|
|||||||||||||||||||||||
|
|||||||||||||||||||||||
| Mad City Labs | Z100/Z500 Piezo stages | H301-MCL-Z100/500 | - | ||||||||||||||||||||
| Marzhauser | Scan IM series (110x160mm opening) | H301-K-FRAME | - | ||||||||||||||||||||
|
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|
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|
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|
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| Thorlabs | Thorlabs MLS203-1 /MLS203-2 | H301-K-FRAME | THORPA | ||||||||||||||||||||
|
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| Upright Microscopes | any stage for uprightmicroscopes | H301-UP | - | ||||||||||||||||||||
Stage top incubator's controller for application with pre-mixed gas.
UNO-T-H-PREMIXED is an all-in-one stage top incubator's controller for application with pre-mixed gas, that includes:
Stage top incubator's controller for application with 100% CO2 and background Air.
UNO-T-H-CO2 is an all-in-one stage top incubator's controller with CO2/Air mixer, for application with 100% CO2 and background Air, that includes:
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